| Rune Name | Rune Location | |
| Vine Rune |  |
Promenade of the Condemned |
| Teleportation Rune |  |
Toxic Sewers |
| Challenger Rune |  |
The Black Bridge |
| Belier’s Rune |  |
Ossuary |
| The Spider’s Rune |  |
Slumbering Sanctuary |
Vine Rune
Where to find: Promenade of the Condemned
Ability: Creates a vine from green growths on the ground, allowing you to access up high areas
Unlocked Zones: Toxic Sewers
Teleportation Rune
Where to find: Toxic Sewers
Ability: Allows you to teleport between the purple coffins
Unlocks: Ossuary, Forgotten Sewers
Challenger Rune
Where to find: The Black Bridge, defeat The Incomplete One
Ability: Opens daily run challenges!
Unlocks: Opens daily run challenges!
Belier’s Rune
Where to find: Ossuary, in this room
Ability: Breaks through the ground at specially marked runes
Unlocks: Old Sewers
The Spider’s Rune
Where to find: Slumbering Sanctuary
Ability: Wall climb!